Production of Bioactive Enzyme L-asparaginase from Fungal Isolates of Water Sample through Submerged Fermentation
نویسندگان
چکیده
L-asparaginase is used in the treatment of cancer, especially for acute lymphoblastic leukemia. Fungal species were isolated from water sample and screened for its ability to produce the extracellular L-asparaginase enzyme. Effect of pH and temperature were investigated in the production of enzyme. Highest yield of enzyme was observed when the pH of the fermentation cycle raised above 8 at 35°C. Effective production was observed while using starch and proline as carbon and nitrogen source. The molecular weight of L-asparaginase was determined by SDS-PAGE & it was found to be 48 kDa.
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